Chicken Interleukin 8 (IL-8) ELISA kit
Product Details
The kit is for the quantitative level ofIL-8 in the sample, adopt purified Chicken IL-8 to coat microtiter plate, make solid-phase antibody, then addIL-8 to wells, Combine IL-8 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of IL-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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